Evaluation of Herbicide Sensitivity and Resistance in Cold-Hardy Grapevine Cultivars
Status: Planned - Updates will be posted at the bottom of the protocol page here
1. Objective
To quantify the visual injury, physiological response, and recovery rates of various cold-hardy white and red grapevine cultivars when exposed to simulated drift concentrations of Dicamba, Roundup (Glyphosate), and 2,4-D.
2. Background / Rationale
Herbicide drift from neighboring row-crop operations remains a primary constraint for vineyard establishment and longevity in prairie regions. While the sensitivity of traditional vinifera is well-documented, there is limited peer-reviewed data regarding the specific resistance levels of newer cold-hardy hybrids like Clarion or Crimson Pearl under prairie conditions.
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Observations: Frequent grower reports of significant damage from neighboring soybean or corn applications.
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Need: Identifying "low-sensitivity" cultivars could influence future vineyard site selection and cultivar choice for high-risk areas.
3. Hypothesis
Cultivars will exhibit distinct, measurable levels of resistance and recovery based on their genetic parentage.
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Systemic herbicides (Roundup) will cause more terminal shoot death.
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Growth-regulator herbicides (2,4-D and Dicamba) will produce varying levels of leaf strapping and cupping.
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Certain hybrids will demonstrate significantly faster recovery of vigor compared to more sensitive varieties.
4. Cultivars Tested
White Cultivars : La Crescent, Brianna, Edelweiss, Itasca, Prairie Star, Frontenac Blanc, Vignoles, Clarion
Red Cultivars: Frontenac, Marquette, Petite Pearl, Crimson Pearl, St. Croix, Verona
5. Experimental Design
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Treatment Groups: Vines will be exposed to ultra-low "drift-level" concentrations (e.g., 1/100th or 1/1000th of field rate) of each herbicide.
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Control Group: Vines receiving only standard vineyard maintenance.
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Layout: Randomized block design to ensure each cultivar is represented in both treatment and control groups.
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Site Documentation: USDA Zone, soil type (Loamy Clay), and high-wind exposure characteristics will be recorded.
6. Treatment Application
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Timing: Application will occur during the rapid shoot growth phase (pre-bloom) when vines are most sensitive to hormone-based herbicides.
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Method: Targeted hand-sprayer application to simulate drift patterns rather than direct over-the-top spraying.
7. Data Collected
Herbicide Injury Assessment
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Visual Rating Scale: 0 (No injury) to 10 (Vine death).
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Symptom Tracking: Specific documentation of leaf cupping (Dicamba), strapping (2,4-D), and chlorosis/terminal yellowing (Roundup).
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Recovery Rate: Weekly monitoring of new growth to determine if the vine "grows out" of the injury or suffers prolonged stunting.
Growth Metrics
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Shoot Elongation: Comparison of shoot length between treated and control vines.
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Fruit Set Impact: Observations on cluster abortion or poor fruit set following exposure.
8. Timing
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Late Spring: Baseline vigor measurements and initial treatment application.
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Early Summer: Weekly injury ratings and recovery observations.
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Post-Harvest: Evaluation of cane maturation and potential impact on winter hardiness.
9. Limitations
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Drift Simulation: Micro-applications may not perfectly replicate the dynamics of real-world chemical drift.
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Environmental Interaction: Temperature and humidity at the time of exposure can significantly alter herbicide volatility and uptake..
10. Sensitivity Protocol (Post-Exposure)
Following exposure, the following "Vulnerability Table" will be used to categorize cultivar response: